Dot Blot: Unmodified and various modified cytosine DNA were probed Anti-5-Hydroxymethylcytosine (5hmC) (1:5000 dilution). Proteins were visualized using a Goat Anti-Mouse IgG secondary antibody conjugated to HRP and a chemiluminescence detection system
Supplier Page from United States Biological for Rat Anti-5-Hydroxymethylcytosine Antibody (MaxLight™650)